FMDV NSP Antibody 3BAC ELISA Test Kit

Principle

Foot-and-mouth disease virus (FMDV) Non-structural protein Antibody 3ABC ELISA Test Kit is suitable for test serum of cattle, sheep, goats and pigs, it can distinguish between immunized animals and wild-infected animals.

This kit uses block ELISA method, FMDV-3ABC antigen is pre-coated on enzyme micro-well strips. When testing, add diluted serum sample, after incubation, if there is FMD virus specific antibody, it will combine with the pre-coated antigen, discard the uncombined antibody and other components with washing; then add enzyme labeled anti-FMD virus monoclonal antibody, antibody in sample block the combination of monoclonal antibody and pre-coated antigen; discard the uncombined enzyme conjugate with washing; Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample, use ELISA reader at 450nm wave length to measure the absorbance A value in reaction wells after adding stop solution to stop the reaction.