Infectious Bronchitis(IB) IgG ELISA Test Kit

Introduction

The Infectious Bronchitis IgG (IBV) antibody ELISA kit is developed to detect the IBV antibodies level in chicken serum sample and can be used to evaluate serological diagnosis of infected chickens and Epidemiological surveys of Infectious Bronchitis virus, analysis of Infectious Bronchitis virus vaccine status in chickens. 

Principle

This kit is based on solid-phase enzyme-linked immunosorbent assay (ELISA) principle, composed by the reaction Micro-plate coated with high purity IBV antigen, horseradish peroxidase-labeled anti-chicken IgG and other reagents. The reaction mechanism is the coated antigen binding with IBV-Ab in sample, and then with the enzyme-labeled anti-chicken IgG antibody to form a "coated antigen + IBV-Ab + anti-chicken IgG HRP antibody" complex, add substrate, it will have coloration by the enzyme catalytic reaction. Color depth is proportional to the amount of IBV-Ab, when the sample chromogenic reaction, the results detected by the microplate reader exceeds a set threshold value result judged as positive, indicating that the immune produced antibodies or natural infection exists.