Foot and Mouth Disease Virus Type A Antibody ELISA Test Kit for Bovine and Ovine

Principle

This kit is used to detect specific antibody against foot and mouth disease virus (FMDV) Type A Antibody in bovine and goat serum qualitatively, for monitoring antibody after FMDV Type A immune.

This kit uses indirect ELISA method, purified FMDV antigen is pre-coated on enzyme micro-well strips. When testing, add diluted serum sample, after incubation, if there is FMD virus specific antibody, it will combine with the pre-coated antigen, discard the uncombined antibody and other components with washing; then add enzyme labeled anti-FMD virus monoclonal antibody, antibody in sample block the combination of monoclonal antibody and pre-coated antigen; discard the uncombined enzyme conjugate with washing; Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample, use ELISA reader at 450nm wavelength to measure the absorbance A value in reaction wells after adding stop solution to stop the reaction.