Echinoccocosis Dog Feces Antigen ELISA Kit

Principle

The test kit is enzyme immunoassay for the detection of Echinoccocosis antigen in dog feces sample. The purified dog Echinoccocosis antibody are coated on 96-well plate, add sample into wells and then antibodies bound specifically by antigens, the unbound antibodies and other components will be removed by washing. Next add enzyme conjugation to specifically bind with the compound of antibodies and antigens on the microplate. The unbound enzyme conjugation will be removed by washing. Add the TMB substrate in the well, react to a blue product. At last, end the reaction by adding stop solution. Use ELISA reader at 450nm wavelength to test the OD value, compare with CUTOFF value, then detect whether the Echinoccocosis antigen is existed in sample.