Sulfamethazine (SM2) ELISA Test Kit

Principle

The test kit is based on the competitive enzyme immunoassay for the detection of Sulfamethazine(SM2) in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Sulfamethazine in the sample and the conjugated antigen pre-coated on the micro-well stripes compete for the anti-Sulfamethazine antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Sulfamethazine concentration in the sample. This value is compared to the standard curve and the Sulfamethazine concentration is subsequently obtained.