Cyproheptadine ELISA Test Kit

Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Cyproheptadine in urine, tissue sample. The coupling antigens are pre-coated on the micro-well stripes. The Cyproheptadine in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Cyproheptadine antibody, add TMB substrate for coloration. The higher Cyproheptadine in the sample, the less ELISA antibody combined at the solid phase, the lighter of the coloration. The optical density (OD) value of the sample has a negative correlation with the Cyproheptadine in it. This value is compared to the standard curve and the Cyproheptadine concentration is subsequently obtained.