One Step Test for Mb

Principle

This test kit uses double antibody sandwich method, to detect Myoglobin (Mb) level in sample. The purified Mb coated on NC membrane. When testing, put sample into buffer and mix it evenly, there is fluorescein labeled Mb antibody in buffer. If there is Mb antibody in sample, it can combine with fluorescein labeled Mb antibody in buffer, form a complex, affected by chromatography, it reacts with coated Mb antibody when coming to the place which coated Mb antibody. If no Mb antibody in sample, then no reaction. When the reaction over, put the test card into Fluorescence Spectrometer, calculate the Mb antibody concentration by the strength of the fluorescence signal.