Infectious Bursal Disease(IBD) Antibody ELISA Test Kit

Introduction

The Avian Infectious Bursal Disease virus VP2 antibody (IBDV-VP2 Ab) ELISA kit is developed to detect the IBDV-VP2 antibodies level in chicken serum sample and can be used to evaluate Infectious Bursal Disease vaccine status in chickens.

Principle

This kit is based on solid-phase enzyme-linked immunosorbent assay (ELISA) principle, composed by the reaction Micro-plate coated with high purity IBDV-VP2 antigen, horseradish peroxidase-labeled anti-chicken IgG and other reagents. The reaction mechanism is the coated antigen binding with IBDV-VP2-Ab in sample, and then with the enzyme-labeled anti-chicken IgG antibody to form a "coated antigen + IBDV-VP2-Ab + anti-chicken IgG HRP antibody" complex, add substrate, it will have coloration by the enzyme catalytic reaction. Color depth is proportional to the amount of IBDV-VP2-Ab, when the sample chromogenic reaction, the results detected by the microplate reader exceeds a set threshold value result judged as positive, indicating that the immune produced antibodies or natural infection exists.