Toxoplasma gondii Antibody ELISA Kit for Bovine, Sheep

Principle

This kit uses indirect ELISA method, TOX antigen is pre-coated on enzyme micro-well strips. When testing, add diluted serum sample, after incubation, if there is TOX specific antibody, it will combine with the pre-coated antigen, discard the uncombined antibody and other components with washing; then add enzyme labeled anti-TOX virus antibody, combine with specific antigen-antibody complex; discard the uncombined enzyme conjugate with washing; Add TMB substrate in micro-wells, there will be blue product, use ELISA reader at 450nm wavelength to measure the absorbance A value in reaction wells after adding stop solution to stop the reaction.